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ACT® Science Example Questions & Practice Problems

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Prepare for the ACT® Science section with realistic, exam-style practice problems that strengthen your critical thinking and data analysis skills. You can create unlimited customized practice sets, review in-depth explanations, and track your progress to see your score improve fast.

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Free Example ACT Science Questions

UWorld’s ACT Science practice problems mirror the actual exam in both format and difficulty. Not getting something? Our visual aids and in-depth answer explanations make hard stuff easy to understand. Experience these science ACT practice problems firsthand and see how realistic exam prep can be.

Passage

The plains zebra (Equus quagga) is a large, grazing equid (member of the horse family) found in much of southern Africa. Although it is assumed that the coloration of the zebra's coat is an evolutionary adaptation, the functional significance of the pattern of black and white stripes, which varies from animal to animal, is not well understood. Consider the following 3 hypotheses regarding the selective advantage provided by the stripes with respect to protection against predators and biting, disease-carrying flies.

Hypothesis 1

Zebras are protected by their stripes from predators and from biting flies. The black and white stripes provide camouflage from predators by breaking up the outline of the zebra against its background. Stripes protect zebras from biting flies because the flies are less likely to land on striped surfaces than they are to land on solid-colored surfaces.

Hypothesis 2

Zebras are protected by their stripes from predators but not from biting flies. The black and white stripes camouflage the zebra when it is stationary. Additionally, the stripes produce an optical illusion that confuses predators when the zebra is in motion. Because flies are attracted to dark colors, the black stripes on the zebra attract biting flies even though the white stripes are present.

Hypothesis 3

Zebras are protected by their stripes from biting flies but not from predators. Zebra predators such as lions and hyenas often hunt at night. At night, the zebra's white stripes remain visible to predators and do not help conceal the zebras. Because flies use vision to locate and land on their hosts, they are active only during the daytime. The white stripes protect the zebra from biting flies by interrupting the dark-colored coat to which the flies are attracted.

Question

According to Hypothesis 3, which of the following observations provides the strongest evidence that the presence of black and white stripes does NOT protect zebras from lions and hyenas?

A. Black stripes are not visible to lions and hyenas under low-light conditions.
B. White stripes are visible to lions and hyenas under low-light conditions.
C. Lions and hyenas often hunt at night.
D. Zebras are not the only large grazing animals preyed upon by lions and hyenas.

Explanation

Hypothesis 3: At night, the zebra's white stripes remain visible to the predators and do not help conceal the zebras.

Analyze Hypothesis 3 to determine which answer choice provides the strongest evidence that the presence of black and white stripes does NOT protect zebras from lions and hyenas.

According to Hypothesis 3:

  • A zebra's white stripes are visible to predators at night.
  • The stripes do not help conceal the zebra.

Therefore, the observation that provides the strongest evidence that the presence of black and white stripes does NOT protect zebras from lions and hyenas is that white stripes are visible to lions and hyenas under low-light conditions such as at night.

(Choice A) An observation that black stripes are not visible to lions and hyenas under low-light conditions would provide evidence that the black stripes do protect zebras from lions and hyenas, which contradicts the hypothesis.

(Choices C and D) The observations that lions and hyenas often hunt at night and prey upon animals other than zebras do not help determine whether black and white stripes protect zebras from lions and hyenas.

Things to remember:
To determine which observation provides the strongest evidence that supports a claim, identify the observation that is directly related to the claim and is logically consistent with the claim.

Passage

Phthalates are chemicals that are added to plastics to make them soft and flexible. Because phthalates are not chemically bound to the plastics to which they are added, they can be released into the environment. Two experiments examined how the shape and concentration of iron oxide (Fe2O3) nanoparticles affect the degradation (chemical breakdown) of environmental dibutyl phthalate (DBP) upon exposure to light and hydrogen peroxide (H2O2).

Experiment 1

Each of 15 identical beakers received 100 mL of a DBP solution with a concentration of 13 mg/L. The beakers were equally divided into 5 groups (Groups J–N). For each group of beakers, Fe2O3 nanoparticles with 1 of 5 different shapes were added to achieve a nanoparticle concentration of 0.30 mg/mL (see Table 1).

Table 1

Each beaker then received 50 microliters (µL) of H2O2 and was placed on a magnetic stirrer located 20 cm from a 250 W light source. After 60 min, the concentration of DBP in each beaker was measured, and the average percent DBP degraded in each group was calculated (see Figure 1).

Bar chart of average DBP degraded

Figure 1

Experiment 2

Each of 15 identical beakers received 100 mL of a DBP solution with a concentration of 13 mg/L. The beakers were then equally divided into 5 groups (Groups O–S). Each beaker received 50 µL of H2O2 and was placed on a magnetic stirrer located 20 cm from a 250 W light source. For each group of beakers, hollow-shaped Fe2O3 nanoparticles were then added at 1 of 5 different nanoparticle concentrations (see Table 2).

Table 2

After 60 min, the average percent DBP degraded in each group was determined as in Experiment 1 (see Figure 2).

Bar chart of average DBP degraded

Question

Consider the units of measurement "µL" and "mg/mL" in Experiment 2. Which of the following phrases best describes the quantities represented by these units?

µL mg/mL
A. mass of H2O2 duration of nanoparticle exposure
B. mass of H2O2 nanoparticle concentration
C. volume of H2O2 duration of nanoparticle exposure
D. volume of H2O2 nanoparticle concentration

Explanation

Experiment 2: Each beaker received 50 µL of H2O2…. For each group of beakers, hollow shaped Fe2O3 nanoparticles were then added at 1 of 5 different nanoparticle concentrations (see Table 2).

Table 2

Read the passage and analyze the results of Experiment 2, shown in Table 2, to determine which choice best describes the quantities represented by "µL" and "mg/mL."

According to the passage, 50 µL of H2O2 was added to each beaker in Experiment 2.

flask Prior Knowledge: Units that involve liters, including microliters (millionth of a liter) typically are a measure of volume.

Table 2 shows that the unit for nanoparticle concentration in Experiment 2 is "mg/mL."

Therefore, the phrase that best describes the quantity represented by "µL" is the volume of H2O2, and the phrase that best describes the quantity represented by "mg/mL" is the nanoparticle concentration.

(Choices A and B) "µL" is a unit of volume, not mass.

(Choice C) Although "µL" is the unit representing the volume of H2O2, the unit "mg/mL" represents the concentration of the nanoparticles, not the duration of exposure to the nanoparticles.

Things to remember:
The quantities represented by particular units can be determined by using information given in the passage about the quantities and by obtaining information from the figures.

Passage

A study was done to examine the rate at which petroleum hydrocarbons (PHC) from crude oil are degraded by microorganisms in soil from 4 geographical regions: Dagang (DG), Bohai Sea (BS), Northeast China (NE), and Xiamen (XM). First, for each region 25 g of crude oil was mixed with 500 g of soil in a plastic container. The containers were then incubated in the dark at 22°C for 112 days. Figure 1 shows how the PHC concentration, in g/kg, in each soil sample changed over time. Table 1 shows the pH and the percent by mass of clay, silt, and sand, respectively, of each of the 4 soils, prior to the addition of the crude oil.

Chart showing PHC concentration in soil

Figure 1

Soil region table

Table 1

1. This work "Bioremediation" is a derivative of "Aerobic degradation of crude oil by microorganisms in soils from four geographic regions of China" by Q. Liu et al., published under CC 4.0 by Scientific Reports (2017). This work, "Bioremediation," is licensed by UWorld.

Question

Which of the following statements comparing the PHC concentration in NE soil with the PHC concentration in XM soil is supported by the results in Figure 1?

A. For all the days after Day 0, the PHC concentration in NE soil was greater than the PHC concentration in XM soil.
B. For all the days after Day 0, the PHC concentration in NE soil was less than the PHC concentration in XM soil.
C. For all the days after Day 0, the PHC concentration in NE soil was the same as the PHC concentration in XM soil.
D. For some of the days after Day 0, the PHC concentration in NE soil was greater than the PHC concentration in XM soil; on the other day, the PHC concentration in NE soil was less than the PHC concentration in XM soil.

Explanation

Chart showing PHC concentration in soil

Examine Figure 1 to determine which statement comparing the PHC concentration in NE soil with the PHC concentration in XM soil is supported by the data.

The height of each bar represents the PHC concentration in soil. Figure 1 shows:

  • At Days 7, 28, and 56, the bars representing PHC concentrations in NE soil are taller than the bars representing PHC concentrations in XM soil.

  • At Day 112, the bar representing PHC concentration in NE soil is shorter than the bar representing PHC concentration in XM soil.

Based on this information, the statement "For some of the days after Day 0, the PHC concentration in NE soil was greater than the PHC concentration in XM soil; on the other day, the PHC concentration in NE soil was less than the PHC concentration in XM soil" is supported by the results in Figure 1.

(Choices A and B) The PHC concentration in NE soil was not consistently greater than or less than the PHC concentration in XM soil.

(Choice C) The PHC concentration in NE soil was not the same as the PHC concentration in XM soil for any of the days after Day 0.

Things to remember:
A statement that is supported by the results in a particular figure will accurately describe the results shown in the figure.

Passage

Phthalates are chemicals that are added to plastics to make them soft and flexible. Because phthalates are not chemically bound to the plastics to which they are added, they can be released into the environment. Two experiments examined how the shape and concentration of iron oxide (Fe2O3) nanoparticles affect the degradation (chemical breakdown) of environmental dibutyl phthalate (DBP) upon exposure to light and hydrogen peroxide (H2O2).

Experiment 1

Each of 15 identical beakers received 100 mL of a DBP solution with a concentration of 13 mg/L. The beakers were equally divided into 5 groups (Groups J–N). For each group of beakers, Fe2O3 nanoparticles with 1 of 5 different shapes were added to achieve a nanoparticle concentration of 0.30 mg/mL (see Table 1).

Table 1

Each beaker then received 50 microliters (µL) of H2O2 and was placed on a magnetic stirrer located 20 cm from a 250 W light source. After 60 min, the concentration of DBP in each beaker was measured, and the average percent DBP degraded in each group was calculated (see Figure 1).

Bar chart of average DBP degraded

Figure 1

Experiment 2

Each of 15 identical beakers received 100 mL of a DBP solution with a concentration of 13 mg/L. The beakers were then equally divided into 5 groups (Groups O–S). Each beaker received 50 µL of H2O2 and was placed on a magnetic stirrer located 20 cm from a 250 W light source. For each group of beakers, hollow-shaped Fe2O3 nanoparticles were then added at 1 of 5 different nanoparticle concentrations (see Table 2).

Table 2

After 60 min, the average percent DBP degraded in each group was determined as in Experiment 1 (see Figure 2).

Bar chart of average DBP degraded

Question

Based on Figure 1, in Group L, what percent of the DBP was NOT chemically broken down after 60 min?

A. 35%
B. 45%
C. 55%
D. 65%

Explanation

P1: Two experiments examined how the shape and concentration of iron oxide (Fe2O3) nanoparticles affect the degradation (chemical breakdown) of environmental dibutyl phthalate.

Table 2

Analyze the passage and Figure 1 to determine the percent of DBP that was NOT chemically broken down after 60 min in Group L.

According to the passage, the degradation of DBP is the chemical breakdown. Thus, the percent DBP degraded is what remains of the initial amount of DBP (100%).

Figure 1 shows that the average percent DBP degraded in Group L was approximately 35%.

Therefore, the percent of the DBP in Group L that was NOT chemically broken down after 60 min was 100% − 35% = 65%.

(Choice A) 35% is the percent of the DBP that was chemically broken down, but the question asks for the percent that was not broken down.

(Choices B and C) 45% and 55% are less than the amount that is not chemically broken down.

Things to remember:
When asked to determine what percent of an event has NOT occurred, subtract the proportion of the event that has occurred from the total.

Passage

The plains zebra (Equus quagga) is a large, grazing equid (member of the horse family) found in much of southern Africa. Although it is assumed that the coloration of the zebra's coat is an evolutionary adaptation, the functional significance of the pattern of black and white stripes, which varies from animal to animal, is not well understood. Consider the following 3 hypotheses regarding the selective advantage provided by the stripes with respect to protection against predators and biting, disease-carrying flies.

Hypothesis 1

Zebras are protected by their stripes from predators and from biting flies. The black and white stripes provide camouflage from predators by breaking up the outline of the zebra against its background. Stripes protect zebras from biting flies because the flies are less likely to land on striped surfaces than they are to land on solid-colored surfaces.

Hypothesis 2

Zebras are protected by their stripes from predators but not from biting flies. The black and white stripes camouflage the zebra when it is stationary. Additionally, the stripes produce an optical illusion that confuses predators when the zebra is in motion. Because flies are attracted to dark colors, the black stripes on the zebra attract biting flies even though the white stripes are present.

Hypothesis 3

Zebras are protected by their stripes from biting flies but not from predators. Zebra predators such as lions and hyenas often hunt at night. At night, the zebra's white stripes remain visible to predators and do not help conceal the zebras. Because flies use vision to locate and land on their hosts, they are active only during the daytime. The white stripes protect the zebra from biting flies by interrupting the dark-colored coat to which the flies are attracted.

Question

Which hypothesis, if any, claims that zebras are protected by their stripes from predators in 2 distinct ways?

A. Hypothesis 1
B. Hypothesis 2
C. Hypothesis 3
D. None of the hypotheses

Explanation

Hypothesis 2: The black and white stripes camouflage the zebra when it is stationary. Additionally, the stripes produce an optical illusion that confuses predators when the zebra is in motion.

Analyze the passage to determine which hypothesis, if any, claims that zebras are protected by their stripes from predators in two distinct ways.

According to the passage, Hypothesis 2 states that the black and white stripes:

  • camouflage a zebra from its predators when the zebra is stationary.
  • produce an optical illusion that confuses predators when the zebra is in motion.

Therefore, Hypothesis 2 claims that zebras are protected by their stripes from predators in two distinct ways.

(Choice A) Hypothesis 1 claims that the black and white stripes provide camouflage for the zebra from its predators. However, it does not claim that the stripes protect the zebra from predators in any other way.

(Choice C) Hypothesis 3 claims that a zebra's stripes do not protect it from predators in any way.

(Choice D) Because Hypothesis 2 describes two ways in which stripes protect zebras from predators, the choice of "none of the hypotheses" is not correct.

Things to remember:
When asked to identify hypotheses that make multiple claims, read the passage and look for specific words associated with the claims.

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Frequently Asked Questions (FAQs)

Yes, our science ACT practice problems match the actual exam in structure, question type, and challenge level.
You get access to hundreds of science ACT practice problems, and you can build unlimited custom practice sets.
Our example ACT Science questions are written by experts and are designed to meet or exceed exam-level difficulty so that the real test feels more manageable.
You can access science ACT practice problems on multiple devices (desktop, laptop, tablet, smartphone). Your progress syncs across all of them.
Our science ACT practice includes passages of all types (Data Representation, Research Summary, Conflicting Viewpoints). Detailed answer explanations and visuals help you decode the harder ones.
Short, timed sessions are best. For example, spend 20–30 minutes each day on focused practice with science ACT practice problems. Track how your speed and accuracy improve.
Many errors come from rushed reading of graphs/data, misinterpreting axes, or neglecting experimental details. A deep review of the explanations for the answers helps you stop repeating those mistakes.
Practicing example ACT Science questions is essential to improve your science score, but you’ll get the biggest boost when reviewing underlying skills: interpreting data, analyzing experiments, and reading visuals. Keep in mind that the science section is now optional, so the science section score won’t help boost your overall score.
Use analytics tools to track your performance by passage type (data vs experiments vs viewpoints), question type (graphs, tables, trend questions), and see which science ACT practice problems you miss most.
No. The science section focuses more on reasoning, data interpretation, and scientific thinking than on heavy memorization. Our example ACT Science questions reflect that focus.
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